La tétracycline, connue sous le nom commercial Sumycin, agit en bloquant la fixation de l’ARNt sur la sous-unité 30S ribosomale, interrompant l’élongation de la chaîne protéique bactérienne. Ce mécanisme confère une activité sur un spectre large, incluant bactéries Gram positives, Gram négatives, rickettsies et spirochètes. Sa biodisponibilité digestive varie selon la prise alimentaire et les interactions avec les ions divalents comme calcium et magnésium. Sa diffusion tissulaire est importante, notamment dans les voies respiratoires et génito-urinaires. L’élimination se fait par voie rénale et biliaire. Les effets indésirables incluent photosensibilisation, troubles digestifs et coloration dentaire en cas d’administration précoce. Les guides thérapeutiques mentionnent sumycin prix, en soulignant la nécessité de restreindre son utilisation afin de limiter les résistances acquises.

Bio-diagnostic.pagesperso-orange.fr

5- INSTRUCTION FOR USE
An antibiogram test is generally performed when a mycoplasma numeration is higher than 10 CCU/ml, as Kit for Genitourinary Mycoplasma
determined with the KITANUM (ref PL10041).
Antibiotic Susceptiblity Test
5.1- Define appropriate number of 2x8 microwells stripsneeded for patient specimens. For one patient, one strip is 6 tests (ref PL10041)
5.2- Place the needed strips on the microwell plate frame.
5.3- According to the actual titer, dilute the inoculatedtransport medium A3 with urea medium U9 (for an 1- INTRODUCTION
infection by Ureaplasma urealyticum) or with arginine medium M42 (for a Mycoplasma hominis infection) as A high level of mycoplasma in specimen (> 10 CCU/ml) is often associated with an inflammatory desorder.
Numeration
Dilute the inoculated transport
In this case, a susceptibility test to Antibiotics should be (in CCU/ml)
In the susceptibility test, inoculum calibration is very important to avoid over- or under-estimmated Minimal Inhibition Concentration. It is the reason why numeration shoud be performed (with the MYCOKIT NUM) before.
3- STORAGE
The test is performed from the inoculated specimen
- The kit is provided at room temperature. upon transport medium A3 stored at +4°C, which was used
reception, the reagents are stable for 1 year at -20°C (or 4 ml of this dilution is needed to perform the test.
to determine the infectious titer in CCU/ml.
until the expiration date).
- Avoid repeated (more than 2-times) freezing and 5.4- Distribute 200 µl of the diluted inoculated transportmedium in each well of the strip.
2- KIT CONTENTS
thawing of the medium.
- After a second thawing, M42 and U9 medium should be 5.5- cover the microplate with a self-adhesive sheet and - 2x8 microwell coated strips with seven antibiotics at 2 4- SAFETY PRECAUTIONS
6. RESULTS
doxycyclin: 16 and 8 µg/mlerythromycin: 8 and 2 µg/ml Susceptibility to an antibiotics inhibit mycoplasma growth. In this case, the color of the medium do not * Don’t smoke, eat or drink in aeras where reagents and If a mycoplasma strain is resistant to an antibiotic, its growth induce the color change of the medium (which * Wear disposable gloves during manipulation of reagents When the two concentrations of antibiotic inhibit the * Use pipettes with disposable sterilized tips to avoid growth the result is SENSITIVE.
___________________________________________________________________________________________________________________________________________________________
APOH –Technologies : division bio diagnostic – ZA Les Flandres - 98, av. de Choisy – 94190 Villeneuve Saint Georges – France – Tél : 33 (0) 1 43 86 82 06 – Fax : 33 (0) 1 43 86 82 09
When only the highest concentration inhibits the growth
result is INTERMEDIATE.
When growth is observed at both concentrations the result
is RESISTANT.
Manufactured by :
APOH-Technologies : division bio diagnostic www.bio-diagnostic.fr ; e-mail : info@bio-diagnostic.fr tel : 33 (0) 1 43 86 82 06 - fax : 33 (0) 1 43 86 82 09 ___________________________________________________________________________________________________________________________________________________________
APOH –Technologies : division bio diagnostic – ZA Les Flandres - 98, av. de Choisy – 94190 Villeneuve Saint Georges – France – Tél : 33 (0) 1 43 86 82 06 – Fax : 33 (0) 1 43 86 82 09

Source: http://bio-diagnostic.pagesperso-orange.fr/images/eng.ATB.pdf