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5- INSTRUCTION FOR USE
An antibiogram test is generally performed when a mycoplasma numeration is higher than 10 CCU/ml, as Kit for Genitourinary Mycoplasma
determined with the KITANUM (ref PL10041).
Antibiotic Susceptiblity Test
5.1- Define appropriate number of 2x8 microwells stripsneeded for patient specimens. For one patient, one strip is 6 tests (ref PL10041)
5.2- Place the needed strips on the microwell plate frame.
5.3- According to the actual titer, dilute the inoculatedtransport medium A3 with urea medium U9 (for an 1- INTRODUCTION
infection by Ureaplasma urealyticum) or with arginine medium M42 (for a Mycoplasma hominis infection) as A high level of mycoplasma in specimen (> 10 CCU/ml) is often associated with an inflammatory desorder.
Numeration
Dilute the inoculated transport
In this case, a susceptibility test to Antibiotics should be (in CCU/ml)
In the susceptibility test, inoculum calibration is very important to avoid over- or under-estimmated Minimal Inhibition Concentration. It is the reason why numeration shoud be performed (with the MYCOKIT NUM) before.
3- STORAGE
The test is performed from the inoculated specimen
- The kit is provided at room temperature. upon transport medium A3 stored at +4°C, which was used
reception, the reagents are stable for 1 year at -20°C (or 4 ml of this dilution is needed to perform the test.
to determine the infectious titer in CCU/ml.
until the expiration date).
- Avoid repeated (more than 2-times) freezing and 5.4- Distribute 200 µl of the diluted inoculated transportmedium in each well of the strip.
2- KIT CONTENTS
thawing of the medium.
- After a second thawing, M42 and U9 medium should be 5.5- cover the microplate with a self-adhesive sheet and - 2x8 microwell coated strips with seven antibiotics at 2 4- SAFETY PRECAUTIONS
6. RESULTS
doxycyclin: 16 and 8 µg/mlerythromycin: 8 and 2 µg/ml Susceptibility to an antibiotics inhibit mycoplasma growth. In this case, the color of the medium do not * Don’t smoke, eat or drink in aeras where reagents and If a mycoplasma strain is resistant to an antibiotic, its growth induce the color change of the medium (which * Wear disposable gloves during manipulation of reagents When the two concentrations of antibiotic inhibit the * Use pipettes with disposable sterilized tips to avoid growth the result is SENSITIVE.
___________________________________________________________________________________________________________________________________________________________
APOH –Technologies : division bio diagnostic – ZA Les Flandres - 98, av. de Choisy – 94190 Villeneuve Saint Georges – France – Tél : 33 (0) 1 43 86 82 06 – Fax : 33 (0) 1 43 86 82 09
When only the highest concentration inhibits the growth
result is INTERMEDIATE.
When growth is observed at both concentrations the result
is RESISTANT.
Manufactured by :
APOH-Technologies : division bio diagnostic www.bio-diagnostic.fr ; e-mail : info@bio-diagnostic.fr tel : 33 (0) 1 43 86 82 06 - fax : 33 (0) 1 43 86 82 09 ___________________________________________________________________________________________________________________________________________________________
APOH –Technologies : division bio diagnostic – ZA Les Flandres - 98, av. de Choisy – 94190 Villeneuve Saint Georges – France – Tél : 33 (0) 1 43 86 82 06 – Fax : 33 (0) 1 43 86 82 09

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