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IMMEDIATE HOT LINE: Effective March 5, 2012 This Hot Line is published by ARUP Laboratories to notify clients of updates to our test menu. New tests, inactivatedtests, and test changes will be included in the Hot Line, which is published twice monthly, as needed. Hot Lines and the up-to-datmay also be viewed on our Web site at For additional information, contact ARUP Client Services at (800) 522-2787. Changes areindicated by the red type. Note that only amended fields of an assay appear in this publication. All other fields remain the same.A red check mark () indicates changes that also apply to other tests. Unless otherwise indicated, the tests updated in this Hot Line are referred outside of ARUP Laboratories and reflect the changes made by the laboratory where specimens are sent for testing. MEDICARE COVERAGE OF LABORATORY TESTING
Please remember when ordering laboratory tests that are bil ed to Medicare/Medicaid or other federal y funded programs, the following Only tests that are medical y necessary for the diagnosis or treatment of the patient should be ordered. Medicare does not pay for screening tests except for certain specifical y approved procedures and may not pay for non-FDA approved tests or those If there is reason to believe that Medicare wil not pay for a test, the patient should be informed. The patient should then sign an Advance Beneficiary Notice (ABN) to indicate that he or she is responsible for the cost of the test if Medicare denies payment. The ordering physician must provide an ICD-9 diagnosis code or narrative description, if required by the fiscal intermediary or Organ- or disease-related panels should be bil ed only when al components of the panel are medical y necessary. Both ARUP- and client-customized panels should be bil ed to Medicare only when every component of the customized panel is Medicare National Limitation Amounts for CPT codes are available through the Centers for Medicare & Medicaid Services (CMS) or its intermediaries. Medicaid reimbursement wil be equal to or less than the amount of Medicare reimbursement. The CPT Code(s) for test(s) profiled in this bulletin are for informational purposes only. The codes reflect our interpretation of CPT coding requirements, based upon AMA guidelines published annual y. CPT codes are provided only as guidance to assist you in bil ing. ARUP strongly recommends that clients reconfirm CPT code information with their local intermediary or carrier. CPT coding is the sole responsibility of the The regulations described above are only guidelines. Additional procedures may be required by your fiscal intermediary or carrier. Chloroquine, Plasma
CHLOROQUI

Specimen Required: Collect: Lavender (EDTA) or pink (K2EDTA).
Specimen Preparation: Separate plasma from cells within 2 hours of draw. Transfer 1 mL plasma to an ARUP Standard Transport Tube. (Min: 0.4 mL) Storage/Transport Temperature: Refrigerated. Also acceptable: Room temperature or frozen. Unacceptable Conditions: Separator tubes. Stability (collection to initiation of testing): Ambient: 1 month; Refrigerated: 1 month; Frozen: 1 month
HOT LINE NOTE: Remove information found in the Note field.
Colorado Tick Fever Antibodies, IgG&IgM, IFA
COLOR TICK

Specimen Required: Collect: Plain red.
Specimen Preparation: Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.25 mL) Storage/Transport Temperature: Refrigerated. Stability (collection to initiation of testing): Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 1 month IMMEDIATE HOT LINE: Effective March 5, 2012 Corticotropin Releasing Hormone

Methodology:

Specimen Required: Collect: Green (sodium heparin).
Specimen Preparation: Transport 3 mL plasma. (Min: 1.1 mL) Storage/Transport Temperature: Refrigerated. Stability (collection to initiation of testing): Ambient: 4 days; Refrigerated: 8 days; Frozen: 3 months
HOT LINE NOTE: Remove information found in the Unacceptable Conditions field.
Diuretic Survey, Serum or Plasma
DIURETIC

Specimen Required: Collect: Plain red or lavender (EDTA).
Specimen Preparation: Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.7 mL) Storage/Transport Temperature: Refrigerated. Unacceptable Conditions: Separator tubes. Stability (collection to initiation of testing): Ambient: 1 week; Refrigerated: 1 week; Frozen: 6 months
HOT LINE NOTE: Remove information found in the Remarks field.
Febrile Seizures Panel
FEBRIL PAN

CPT Code(s):
83891 Isolation, 83892 Digestion, 83896 x26 Nucleic acid probes, 83898 x90 Amplification, 83900 Multiplex amplification, 83901 x24 Amplification, 83904 x90 Sequencing, 83909 x91 Capillary electrophoresis, 83912 Interpretation and report FLT3 Mutation Detection by PCR
FLT3 MUTAT

Specimen Required: Collect: Lavender (EDTA), yellow (ACD solution A or B), or green (sodium or lithium heparin).
Specimen Preparation: Transport 5 mL whole blood (Min: 4 mL) OR 3 mL bone marrow. (Min: 1 mL) Separate specimens must be
submitted when multiple tests are ordered.
Storage/Transport Temperature: Refrigerated. Unacceptable Conditions: Serum or plasma. Frozen specimens. Clotted whole blood. Severely hemolyzed specimens. Stability (collection to initiation of testing): Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable Fluoride, Urine
FLUORIDE U

Specimen Required: Collect: Urine collected in a trace metal-free or acid-washed plastic container.
Specimen Preparation: Transport 6 mL urine. (Min: 2.9 mL) Submit in ARUP Trace Element-Free Transport Tubes (ARUP supply #43116). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. Storage/Transport Temperature: Refrigerated. Remarks: Avoid exposure to gadolinium-based contrast media for 48 hours prior to specimen collection. Stability (collection to initiation of testing): Ambient: 1 week; Refrigerated: 1 week; Frozen: 1 month New Test 2006328
Glutathione Total

Methodology:
Performed:
Reported:

Specimen Required: Collect: Yellow top (ACD Solution B).
Specimen Preparation: Transport 10 mL blood in original collection container. (Min: 1 mL) Storage/Transport Temperature: CRITICAL REFRIGERATED.
Unacceptable Conditions: Hemolyzed specimens. Stability (collection to initiation of testing): Ambient: Unacceptable; Refrigerated: 21 days; Frozen: Unacceptable
Reference Interval: By Report

CPT Code(s):

New York DOH approval pending. Call for status update.
HOT LINE NOTE: Refer to the Test Mix Addendum for interface build information.
IMMEDIATE HOT LINE: Effective March 5, 2012 Glyburide, Serum or Plasma
GLYBURID

Specimen Required: Collect: Plain red or lavender (EDTA).
Specimen Preparation: Transfer 3 mL serum or plasma ton an ARUP Standard Transport Tube. (Min: 1.2 mL) Storage/Transport Temperature: Refrigerated. Unacceptable Conditions: Separator tubes. Stability (collection to initiation of testing): Ambient: 1 week; Refrigerated: 1 week; Frozen: 4 months
HOT LINE NOTE: There is a component change associated with this test that affects interface clients only. Refer to Test Mix Addendum for further
information.
Human Anti-Mouse Antibody (HAMA), ELISA

Specimen Required: Collect: Plain red or serum separator tube.
Specimen Preparation: Transport 1 mL serum. (Min: 0.5 mL) Stability (collection to initiation of testing): Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 1 month Heroin, Serum or Plasma - Screen with Reflex to Confirmation/Quantitation
HEROIN SP

Specimen Required: Collect: Plain red, lavender (EDTA) or pink (K2EDTA). Also acceptable: Gray (sodium fluoride/potassium oxalate).
Specimen Preparation: Separate from cells within 2 hours. Transfer 4 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 1.6 mL) Storage/Transport Temperature: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Unacceptable Conditions: Thawed specimens. Separator tubes. Stability (collection to initiation of testing): Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 6 months HLA Antibody Detection
HLA ABSCN

Performed:
Reported:

Specimen Required: Collect: Plain red.
Specimen Preparation: Transfer 5 mL serum to ARUP Standard Transport Tubes. (Min. 2 mL) Stability (collection to initiation of testing): Ambient: 48 hours; Refrigerated: 1 month; Frozen: 2 years
Interpretive Data:Background Information for HLA Antibody Detection:
Purpose: To detect HLA Class I IgG antibody.
Analytical Sensitivity & Specificity:
More sensitive than conventional lymphocyte cytotoxicity procedures.
Limitations:
Only detects IgG antibody isotype; IgM antibody not detected
Test Results:
Results are reported as panel reactive (PRA) present or absent and do not provide a specificity.
LymphogranulomaVenereum (LGV), Differentiation Antibody Panel, MIF
LYMPH VEN

Specimen Required: Collect: Plain red or serum separator tube.
Specimen Preparation: Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL) Storage/Transport Temperature: Refrigerated. Stability (collection to initiation of testing): Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 1month IMMEDIATE HOT LINE: Effective March 5, 2012 New Test 2006330
Mycoplasma pneumoniae Antibody, IgA

Methodology:
Semi-QuantitativeEnzyme-Linked Immunosorbent Assay Performed:
Reported:

Specimen Required: Collect: Plain red or SST.
Specimen Preparation: Transfer 1 mL serum to an ARUP Standard Transport Tube and freeze immediately. (Min: 0.6 mL) Storage/Transport Temperature: CRITICAL FROZEN.Separate specimens must be submitted when multiple tests are ordered.
Unacceptable Conditions: Thawed specimens. Stability (collection to initiation of testing): Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 4 months
Reference Interval: By Report

CPT Code(s):

New York DOH Approved.
HOT LINE NOTE: Refer to the Test Mix Addendum for interface build information.
Neurokinin A (Substance K)
NEUROKIN

Specimen Required: Patient Preparation: Patient must be fasting 10-12 hours prior to collection. Antacid medication or medications that
may affect intestinal motility should be discontinued, if possible, for at least 48 hours prior to specimen collection. Collect: GI preservative tube. Order ARUP supply #47190 online through eSupply using Connect or contact ARUP Client Services at 800-522-2787. Specimen Preparation: Separate from cells within 1 hour of draw. Transfer 5 mL plasma to an ARUP Standard Transport Tube and freeze immediately. (Min: 1 mL) Storage/Transport Temperature: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Unacceptable Conditions: Thawed specimens. Specimens not collected in GI preservative tube. Stability (collection to initiation of testing): Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 month Neuronal Nuclear Antibody (Hu) by IFA with Reflex to Titer and Western Blot,

CPT Code(s):
86255 ANNA; if reflexed, add 86256 Titer; 84181 Western blot Pancreastatin

Specimen Required: Patient Preparation: Patient must be fasting 10-12 hours prior to collection. Patient should not be on any medications that may
influence insulin levels, if possible, for at least 48 hours prior to collection. Collect: GI preservative tube. Order ARUP supply #47190 online through eSupply using Connect or contact ARUP Client Services at 800-522-2787. Specimen Preparation: Separate from cells within 1 hour of draw and transfer 2 mL plasma to an ARUP Standard Transport Tube and freeze immediately. (Min: 1 mL) Storage/Transport Temperature: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Unacceptable Conditions: Thawed specimens. Specimens not collected in GI preservative tube. Stability (collection to initiation of testing): Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 2months Phenol Exposure, Urine

Specimen Required: Collect: Urine collected at end of work shift.
Specimen Preparation: Transfer 4 mL urine to an ARUP Standard Transport Tube. (Min: 1.9 mL) Storage/Transport Temperature: Refrigerated. Remarks: Preservative-free urine specimens are recommended. Unacceptable Conditions: Urine specimens preserved with Benzoic Acid. Stability (collection to initiation of testing): Ambient: 4 days; Refrigerated: 1 week; Frozen: 1 year IMMEDIATE HOT LINE: Effective March 5, 2012 Plasminogen Activator Inhibitor 1, Activity
*This test is performed at ARUP Laboratories.

The kit vendor has changed for this test.

Performed:

Reported:

Reference Interval: By report
Interpretive Data: Refer to report

Source: http://arupbdm.com/Testing-Information/resources/HotLines_Immediate/March%205,%202012%20Hot%20Line.pdf

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