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Hamilton HPLC - HxSil C8, C18 Reversed Phase HPLC Columns HPLC Application Index Ordering Information
Silica-based columns for general separations:
z Better retention of poorly retained compounds z Separation of neutral, acidic and basic compounds without tailing Product Features
z Two particle sizes: 3, 5, 7, 10 and 12-20um. z Five column internal diameters: 2.1 to 21.5 mm. z Analytical and semiprep/preparative guard columns in 316 stainless steel. What Types of Samples Can HxSil C8, C18 Columns Separate?
Sunscreens in Lip Balm
HxSil C18, 4.6 x 150mm, 5um (P/N 79868)
1. Oxybenzone 2. Octocrylene 3. Octyl Methoxycinnamate 4. Avobenzone (Parsol 1789) 5. Octyl Salicylate Conditions: 85:15 Methanol : Water.
Isocratic. Ambient.
Flow: 1 mL/min.
Injection: 5 µL
Detection: UV at 308nm.
Sunscreen Compounds
HxSil C18, 4.6 x 150mm, 5um (P/N 79868)
1. 2-Hydroxy-4-methoxybenzophenone 2. 2-Ethylhexyl trans-4-methoxycinnamate 3. 2-Ethylhexylsalicylate Conditions:7:1 Acetonitrile : Water.
Isocratic. Ambient.
Flow: 1 mL/min.
Injection: 10µL
Detection: UV at 254nm.
Diphenhydramine in Itch Relief Gel
HxSil C18, 4.6 x 150mm, 5um (P/N 79868)
Conditions:9:1 Methanol : 20mM
Potassium Hydrogen Phosphate pH 7.0.
Isocratic. Ambient.
Flow: 2 mL/min.
Injection: 5µL
Detection: UV at 254nm.
Hamilton HPLC - HxSil C8, C18 Reversed Phase HPLC Columns Diphenhydramine in Itch Relief Gel
In Figure 11 the antihistamine diphenhydramine is separated from other components present in the gel in just over 2 minutes. Easy sample preparation is
characterized by water dilution, and filtration. The compound of interest (diphenhydramine) is an amine base.
Meclizine in Pills
HxSil C18, 4.6 x 150mm, 5um (P/N 79868)
Conditions: 9:1 Methanol : 20mM Potassium
Hydrogen Phosphate pH 7.0. Isocratic.
Ambient.
Flow: 2 mL/min.
Detection: UV at 254 nm
Hamilton HPLC - HxSil C8, C18 Reversed Phase HPLC Columns Meclizine in Motion Sickness Pills
The antiemetic, meclizine is separated from excipients in the motion sickness pills. This amine base drug exhibits excellent symmetry (Tailing Factor of
1.061) on a Hamilton HxSil C-18 column.
Tetracycline by USP Method
Conditions: 680mL 0.1N Ammonium
Oxalate, 270mL Dimethylformamide,
50mL 0.2M Dibasic Ammonium
Phosphate pH 7.6. Isocratic. Ambient.
Flow: 1 mL/min.
Injection: 10 µL
Detection: UV at 280 nm
USP Methods
The Hamilton HxSil C-18 column meets the requirements of a USP L1 column and the HxSil C-8 column meets the requirements for an L7 column. The
application above illustrates the analysis of tetracycline and the two applications below demonstrate the separation of oxytetracycline and tetracycline
using a HxSil C-8 "L7" column and folic acid from methotrexate on a HxSil C-18 "L1" column.
Oxytetracycline and Tetracycline
HxSil C8, 4.6 x 150mm, 5um (P/N 79102)
1. Oxytetracycline 0.5mg/mL 2. Tetracycline 0.5mg/mL Conditions: 680mL 0.1N Ammonium Oxalate,
270mL Dimethylformamide, 50mL 0.2 M Dibasic
Ammonium Phosphate pH 7.6. Isocratic. Ambient.
Flow: 1 mL/min.
Injection: 20 uL
Detection: UV at 280nm.
Folic Acid and Methotrexate by USP Method
1. Folic Acid 100ug/mL 2. Methotrexate 100ug/mL Conditions: 9:1 0.126M Sodium
Phosphate, Dibasic, 0.037M Citric
Acid pH 6.0 : Acetonitrile. Isocratic.
Ambient.
Flow: 1.2 mL/min.
Injection: 10 µL
Hamilton HPLC - HxSil C8, C18 Reversed Phase HPLC Columns Detection: UV at 302nm.
Antibiotics
1. . Ampicillin 2. Oxacillin 3. Dicloxacillin Conditions: 3:1 0.05M Potassium
Phosphate pH 7 : Acetonitrile. Isocratic.
Ambient.
Flow: 2 mL/min.
Injection: 5µL
Detection: UV at 254 nm
Small Molecules
HxSil C18, 4.6 x 150mm, 5um (P/N 79868)
1. Uracil 10ppm 2. Caffeine 10ppm 3. Phenol 10ppm 4. Toluene 10ppm 5. Butylbenzene 100ppm 6. Triphenylene 10ppm 7. Amylbenzene 100ppm 8. Terphenyl 10ppm Conditions: 7:3 Acetonitrile : Water.
Isocratic. Ambient.
Flow: 1 mL/min.
Injection: 50 µL
Detection: UV at 254 nm
Hamilton HPLC - HxSil C8, C18 Reversed Phase HPLC Columns ORDERING INFORMATION
HxSil C8 Reversed Phase HPLC Columns
I.D. x Length
HxSil C8, C18 Guard Columns
Analytical Starter Kits (1 holder, 2 cartridges) Semiprep/Preparative Starter Kit (1 holder, 1 cartridge) Semiprep/Preparative Replacement Cartridges (2/pk) Guard Column Technical Information Column Care and Storage Copyright 1998-2004 Hamilton Company. All Rights Reserved.
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Source: http://www.westernanalytical.com/pdf/hxsil.pdf