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Wbut.ac.in

West Bengal
University of
Technology
Tender Form
Scientific Instruments


Notice No : BIO/FO/11-12/31
Date of Issue : 15th-March-2012
D.D. No. for the Tender Price :

Address:
Tele-Fax No:
BF-142, Sector-I, (033) 2334-1034
Salt Lake City,
Kolkata-700064

Phone No.:
(033) 2321-1345


Tender Paper for Infrastructure Development
Ref: Tender Notice No. BIO/FO/11-12/28 dated: 14-March-2012
a) Name & Address of the Firm:
b) Telephone No:
c) Mobile No.:
d) Fax No:
2. Name of the items for which the Firm is interested:
3. Name of the Proprietors/Partners/Directors etc:
4. Trade License No (With Photocopy):
5. VAT License No:
6. Service Tax Registration No (With Photocopy):
7. Additional Information if the party wants to include:
8. Copies of Testimonials/credentials/certificates regarding services/experience
etc:
Signature with Stamp
Please give sealed quotation for the following Infrastructure work (I Nos) clearly
superscribing the tender notice number on the envelope.

Tender Specification
1. Big Dye Terminator V 3.1 2. Electrophoretic Mobility Shift Assay kit 3. FISH probes for telomere length detection 4. TeloTAGGG Kit 5. Taq DNA Polymerase ( 5Unit per microlitre) 6. dNTP Mix ( 2.5 mM each, PCR Grade) 7. Magnesium Cholride (25 millimolar) PCR Grade 8. 50X SSC Buffer 9. Restriction enzymes a) Bam HI (1000 U) b) KspI / (SacII) c) Hinf I d) Mva I (BstN I) e) Taq I f) Cfo I (Hha I) g) Rsa I h) Hinf I i) Spe I j) BanI 10. Isopropanol 11. Tris Saturated Phenol 12. Proteinase K 13. Colcemid 14. Penicil in Streptomycin 15. Exonuclease I 16. Shrimp Alkaline Phosphate 17. Positively charged Nylon Membrane 18. Ethyl Alcohol, Molecular Grade 19. Methanol 20. Glacial Acetic Acid 21. Nuclease free water 22. DNA Isolation Kit 23. Polyethylene Glycol 24. 1 Kilobase DNA Ladder. 25. RPMI 1640 26. Chloroform 27. Isoamyalcohol 28.Sodium Acetate Buffer, Ph 5.2 29. EDTA Disodium salt solution 30. Giemsa stain 31. Ammonium Persulphate 32. TEMED 33. Agarose 34. Acrylamide 35. Bisacrylamide 36. TAE Buffer 37. TBE Buffer 38. TE Buffer 39. Ethidium Bromide 40. Bromophenol Blue 41. Urea 42. Human Serum Cystatin C ELISA kit 43. Human Serum Homocysteine ELISA kit 44. Human Serum Apolipoprotein E ELISA kit 45. DMEM low glucose medium 46. Hydrocortisone 47. Phorbol myristate acetate 48. Dexamethasone 49. Beta glycerol phosphate 50. Ascorbic acid 51. Sodium pyruvate 52. Proline 53. ITS-primax 54. 3-isobutyl-1-methyl xanthine 55. Indomethacin 56. Rabbit serum 57. Cyclic AMP 58. Butylated hydroxyl anisole 59. B27 60. N2 61. Triton X 62. Accustain 63. Bis-benzamide 64. Baicalin (flavanoid) 65. Nerve growth factor(NGF) 66. Brain derived growth factor (BDNF) 67. Fibronectin 68. Vitronectin 69. RA 70. Collagen IV 71. Hepatocyte growth factor 72. Osmostatin M 73. Mesencult 74. Mesenchymal expansion medium 75. Human serum supplement 76. FGF 77. Primary Antibodies ( Oct 4, nanog, nestin,GFAP,NF-200,Neu-200) 78. Secondary antibodies (FITC anti-mouse IgG) 79. Antibodies CD3,CD7,CD19,CD29,CD31,CD33,CD34,CD44,CD45,CD51,SH-2,SH-3 and human 80. L-Glutamine 81. 5-azacytidine 82. Beta-mercaptoethanol 83. Lycopene 84. Allantoin 85. Glutathione (L) 86. Geraniol 87. Ficoll-paque plus 88. Totarol 89. Foetal Bovine Serum (FBS) 90. Beta-Carotene (D) 91. Astaxanthin (Bioastin™) 92. Citrate phosphate dextrose adenine (CPDA) 93. EDTA 94. DMSO 95. Glycerol 96. Isopropanol 97. CD34 cells- anti human CD34 PE 98. CD38- anti human CD38- FITC 99. CD45- anti human CD45- FITC 100. Mouse IgG isotypes for FITC 101. Mouse IgG isotypes for PE 102. Alpha fetoprotein 103. Propidium iodide 104. Hoechst stain 33342 105. Acridine orange 106. Sodium chloride 107. Potassium chloride 108. Disodium hydrogen phosphate 109. Potassium dihydrogen phosphate 110. IMDM 111. RPMI 112. Amphotericin-B 113. Penstrep 114. Phenolphthalein 115. Polyethylene glycol 116. Pentastarch 117. 7-AAD 118. Taurine 119. Sucrose 120. Methanol 121. Human albumin 122. Dextran-40 123. Annexin-V 124. Alpha-tocopherol acetate 125. Epigallocatechin gallate (EGCG) 126. Baicalin 127. Hydrogen peroxide 128. Trizol 129. Bovine Serum Supplement 130. HIT Serum Substitute 131. BIT Serum Substitute 132. Apo Brdu 133. C-kit 134. VED stabilizer- caspase inhibitor (FMK) 135. Sodium phosphate 136. Sodium azide 137. Beta- NADPH 138. Glutathione reductase enzyme 139. Glutathione free acid, reduced form 140. DL-dithiiothreitol 141. Dithiobis(2-nitrobenzoic acid) 142. Thiobarbituric acid 143. Trichloro acetic acid 144. n-butanol 145. Phosphotungstic acid 146. SDS 147. HCl 148. Phytohemagglutinin 149. Acetic acid 150. Dihydrogen- Dichlorofluorocin-diacetaldehyde (DCF-DA) 151. Potassium phosphate 152. Magnesium chloride 153. HEPES Buffer 154. Calcium chloride 155. Glucose 156. Triton X-100 157. Modified Dulbecco’s PBS (DPBS) 158. Trypan blue 159. Dinitrophenylhydrazine 160. Ethyl acetate 161. 6M Guanidine HCl 162. 1-butanol 163. EasySep Human CD34 selection kit 164. Power SYBR Green PCR master mix 165. Taqman universal PCR master mix
166. SUPERSCRIPT VILO CDNA SYNTHESIS KIT-50 RXN
167. PURELINK RNA MICRO KIT 50 Reactions
168. Nuclease free water
169. DNase I AMP Grade 100 UN
170. RNaseZap
171. RNAlater
172. EDTA acid solution 0.5Mm
173. Glutathione peroxidise activity assay kit
174. MTT assay kit
175. SOD assay kit
176. Methocult
177. Protein carbonylation assay kit
178. LDH assay kit
179. Caspase-3 kit
180. LPO assay kit
181. Protein carbonylation assay kit
182. BCA Protein assay kit
183. Benzo[a]pyrene
184. 100-1000µL tips, 5ml tips
185. 0.1 to 2µL tip
186. 2ml eppendorf
187. Sterile falcon 15mL, 50mL
188.
189. Cotton 190. Handcare sterile plastic gloves 191. Latex gloves 192. Syringe 5 mL, 15 mL, 50 mL 193. Bottles 194. Beakers 195. Latex powder free gloves 196. Tissue culture flasks 25 vented 197. Matrigel 198. Poly D lysine and Poly L lysine coated tissue culture flasks 199. Tissue culture flasks 75 vented 200. 96-well flat bottom microplate 201. 6 and 8 wells tissue culture plates 202. Heparin vials 203. Finnipipette F2 8 channel 204. Micro tip box 200-1000 µl 205. Mask 206. 33mm Millex filters with nylon membrane (0.2µm) 207. Cryo 1 C cooler 208. 1.5ml cryovials 209. Countess cell counting chamber slides with trypan blue 210. Tissue culture test plate 96 well (covered) 211. Fast reaction tubes 0.1µl 212. Fast reaction tubes 0.1ml with caps General conditions: 1. The tenderers are required to deposit the tender forms along with relevant papers (mentioned in the form) in two sealed covers mentioning financial bid and technical bid separately. 2. The university reserves the right to accept or reject any tender without showing any reason. 3. Tender for providing the previous work experience list mentioned in the notice may be submitted in full or in part. 4. Tender fee of Rs. 200/- (non refundable) has to be deposited along with the tender papers. 5. All payments should be made through demand drafts in favour of the 'West Bengal University of Technology' payable at Kolkata. 6. The last date of receiving of tender paper in the office of the Finance Officer is March 20th, 2012 before noon. Opening of tender shall be announced shortly. 7. Incomplete tender shall be summarily rejected. 8. Subcontracting in any form will not be entitled by the University. 9. All legal disputes shal be subject to jurisdiction of Calcutta High Court. ********************************************************************************

Source: http://www.wbut.ac.in/datas/users/0-31-bio.pdf

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