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Pombe1.qxd

Introduction to the Schizosaccharomyces pombe Collection
Background
The formation of a collection of Schizosaccharomyces pombe mutant strains was first suggested by Dr AnwarNasim (National Research Council of Canada) and Prof. Jurg Kohli (Universität Bern, Switzerland) whocirculated in 1985 a list of about 400 strains which could form the basis of such a collection. The formerCurator of the NCYC, Mrs Barbara Kirsop, made an offer to the First International Workshop on Fission Yeast,(held in Calgary, Canada in 1986), to house such a collection at the NCYC. The offer was accepted and Dr AlanCoddington (University of East Anglia, UK) offered to help the NCYC gather together the relevant strainsusing the original Nasim-Kohli list as a basis. In May 1986 Dr Coddington was awarded a grant by the UKScience and Engineering Research Council (SERC) to employ a research associate for one year to accessionand catalogue the strains in collaboration with the NCYC.
Schizosaccharomyces pombe
Schiz.pombe has become the model organism for study of the molecular biology of the eukaryotic cell cycle andhas been used extensively for the study of cell cycle control mechanisms, mating type switching andrecombination. In addition ion and metabolite transport coupled to plasma membrane ATPase andmitochondrial function have also been studied. Vectors have been constructed for the transformation ofSchiz.pombe and it has potential as a host for expression of heterologous eukaryotic DNA. The genome ofSchiz.pombe has three linkage groups to which about 250 markers have been mapped. Because of the smallnumber of these groups it is relatively easy to map a new mutation using mitotic haploidisation and meioticrecombination techniques.
The wild type strains of Schiz.pombe now used in genetics research were originally isolated by Leupold [122]from a culture of Schiz.pombe Lindner str. liquefaciens (Osterwalder) from the Centraalbureau voorSchimmelcultures, Delft, The Netherlands. Two heterothallic strains, 972 h- and 975 h+ and two homothallicstrains, h90 and h40 were found to be present in the culture. 972 h- is very stable and has been the mainbackground strain for mutant isolation. It can be readily crossed with 975 h+ which is less stable andspontaneously mutates to h90 on storage. The other homothallic strain h40 has been lost. Since allSchiz.pombe mutants have been derived from Leupold's original heterothallic strains they have a commongenetic background enabling valid mutant-wild type phenotypic comparisons to be made. This situation is incontrast to many other genetic systems where a variety of progenitor strains have been used as a source ofmutants.
Nomenclature
Nomenclatural rules have been defined by Kohli [123] who also gave a definitive list of all mutants known upto that time. All phenotypic and mapping information concerning the mutants in this collection has been derivedfrom Kohli's paper.
Media and Methods
Methods for strain maintenance and carrying out genetic crosses by random ascospore and tetrad analysis aregiven in the appendix to Gutz et al. [1]. The minimal medium described therein can be conveniently replacedwith yeast nitrogen base without amino acids (Difco). Mapping by mitotic haploidisation and diploidconstruction is described in Gygax and Thuriaux [25] and transformation is described in Beach and Nurse[124].
Chromosome map and allocation to linkage groups
Schiz.pombe has three linkage groups and about 250 genes have been allocated. A recent map is reproduced inthe appendix. A summary of the strategy used to map a new marker is given by Gygax and Thuriaux [25].
Briefly this involves first allocating the marker to one of the three linkage groups by induced mitotichaploidisation as described by Kohli et al. [10] and then narrowing down the exact location by three point crosses with appropriate tester strains preferably by tetrad analysis.
Mitochondrial mutants
A number of mitochondrial mutants are known and a partial map of the mitochondrial genome has beenconstructed (Lang et al. [106]).
Deposit of New Cultures
In order to increase the value of the collection still further the NCYC would be pleased to hear of additionalstrains of present or potential interest and in particular those that are cited in the literature. It may not always bepracticable to accept all strains offered to the collection so depositors are asked to contact the NCYC beforedispatching cultures. Accession forms may be obtained at the same time. Cultures accessioned into the opencollection are listed in the catalogue and databases and are available to the scientific community withoutrestriction.
Publications
NCYC would be pleased to receive reprints of any papers published concerning strains held in the collection.
Acknowledgments
The following are warmly thanked for their contributions to the collection: Dr A Coddington, University of East Anglia, Norwich, UK.
Dr P Fantes, University of Edinburgh, Edinburgh, UK.
Dr A Goffeau, Université de Louvain, Belgium.
Dr H Gutz, Institut für Genetik, Braunschweig, Germany.
Prof. J Kohli, Universität Bern, Switzerland.
Dr A Nasim, National Research Council, Ottawa, Canada.
Prof. P Nurse, Imperial Cancer Research Fund, London, UK.
Dr C Shimoda, Osaka City University, Japan.
Prof. M Yanagida, Kyoto University, Japan.
Dr P Young, Queen's University, Ontario, Canada.
In addition Cinzia Dedi is specially thanked for her excellent work in accessioning and cataloguing thecollection at the NCYC.
Financial support from the Science and Engineering Research Council (SERC) for the establishment of thiscollection is gratefully acknowledged. Use of this section of the Catalogue
All strains are listed giving NCYC number, genotype, map location, gene product / phenotype, references anddepositor's initials. At present the collection comprises the following: Auxotrophs: adenine, arginine, glutamate, histidine, leucine, lysine, methionine, proline, tryptophan, uracil,
aromatic amino acids, phenylalanine and tyrosine, purine degradation and non-utilisation.
Drug and analogue resistance/sensitivity mutations: anisomycin, canavanine, benomyl, cycloheximide,
azaguanidine, fluorouracil, ethionine and trichodermin.
Mutations affecting conjugation, meiosis and sporulation: sporulation, sterility, mating type switching,
meiosis and vegetative iodine reaction mutants.
Cell cycle mutations: cell division cycle, nuclear division arrest, cell division without nuclear division.
Temperature sensitive lethals
Radiation sensitive
Suppressors
Acid and alkaline phosphatase
Membrane ATPase
Mapping strains
Guide to Entries
ade7-413 sup1-35
omnipotent suppressor
Key to the Depositors
Dr A Coddington, University of East Anglia, Norwich, UK.
Dr P Fantes, University of Edinburgh, Edinburgh, UK.
Dr A Goffeau, Université de Louvain, Belgium.
Dr H Gutz, Institut für Genetik, Braunschweig, Germany.
Prof. J Kohli, Universität Bern, Switzerland.
Dr A Nasim, National Research Council, Ottawa, Canada.
Prof. P Nurse, Oxford University, Oxford, UK.
Dr C Shimoda, Osaka City University, Japan.
Prof. M Yanagida, Kyoto University, Japan.
Dr P Young, Queen's University, Ontario, Canada.
LIST OF CULTURES
WILD TYPES
AUXOTROPHS
Arginine
Aromatic amino acid
Glutamate
Histidine
Methionine
Tyrosine-phenylalanine
Purine Degradation and non-Utilisation
Tryptophan
DRUG AND ANALOG RESISTANCE/SENSITIVITY
Anisomycin
Azaguanidine
Canavanine
Cycloheximide
Ethionine
Fluorouracil
Trichodermin
MUTATIONS AFFECTING CONJUGATION, MEIOSIS AND SPORULATION
Mating Type Switching
Sporulation
Sterility
Vegetative Iodine Reaction
CELL CYCLE
Cell Division Cycle
Nuclear Division Arrest
Alteration In Nuclear Structure
Cell Division Without Nuclear Division
Changed Division Response
TEMPERATURE SENSITIVE LETHALS
RADIATION SENSITIVE
SUPPRESSORS
ACID AND ALKALINE PHOSPHATASE
MEMBRANE ATPase
MAPPING STRAINS
Numerical List of Schizosaccharomyces pombe Strains
REFERENCES
For ease of cross reference, references are listed in the same order as in the review article by Kohli, J.
1987 : Genetic nomenclature and gene list of the fission yeast Schizosaccharomyces pombe. Curr.
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5. Ibrahim, M.A.K. and Coddington, A. 1978. Genetic studies on revertants to sensitivity from a cycloheximide resistant strain of Schizosaccharomyces pombe. Molec. Gen. Genet., 162, 213-219.
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52. Toda, T., Nakaseko, Y., Niwa, Osami, N. and Yanagida, M. 1984. Mapping of rRNA genes by integration of hybrid plasmids in Schizosaccharomyces pombe. Curr. Genet., 8, 93-97.
53. Janner, F., Vogeli, G. and Fluri, R. 1980. The antisuppressor strain sin1 of Schizosaccharomyces pombe lacks the modification iso-pentenyladenosine in transfer RNA. J. Mol. Biol., 139, 207-219.
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56. Munz, P., Dorsch-Hasler, K. and Leupold, U. 1983. The genetic fine structure of nonsense suppressors in Schizosaccharomyces pombe. Curr. Genet., 7, 101-108.
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59. Girgsdies, O. 1982. Sterile mutants of Schizosaccharomyces pombe: analysis by somatic hybridization.
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63. Sumner-Smith, M., Hottinger, H., Willis, I., Koch, T.L., Arentzen, R. and Soll, D. 1984. The sup8 tRNA Leu gene of Schizosaccharomyces pombe has an unusual intervening sequence and reduced pairing in the
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Genetic Chromosome Map of Schizosaccharomyces pombe
Reproduced from Molecular Biology and Morphogenesis of Fission Yeast (Ed. A.Nasim, P.Young, and B.F.Johnson) by kindpermission of Academic Press.

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